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P2 labs, Confocal Scanning Laser Microscopy, GC-FID for FAME determination
Methods:
- CMI/CNI determination by modeling (Guillier, L., Nazer, A., and Dubois-Brissonnet, F. (2007). Growth response of Salmonella Typhimurium in presence of natural and synthetic antimicrobials: estimation of MICs from three different models. Journal of Food Protection 70(10), 2243-2250.)
- Bacterial membrane fatty acid determination (Dubois-Brissonnet, F., Naitali, M., Maf, A.A., and Briandet, R. (2011). Induction of fatty acid composition modifications and tolerance to biocides in Salmonella enterica serovar Typhimurium by plant-derived terpenes. Applied and Environmental Microbiology 77(3), 906-910; Dubois-Brissonnet, F., Trotier, E., and Briandet, R. (2016). The Biofilm Lifestyle Involves an Increase in Bacterial Membrane Saturated Fatty Acids. Frontiers in Microbiology 7(1673). doi: 10.3389/fmicb.2016.01673)
- Biofilm formation and tolerance to disinfectants (Bridier, A., Dubois-Brissonnet, F., Greub, G., Thomas, V., and Briandet, R. (2011). Dynamics of biocide action in Pseudomonas aeruginosa biofilms. Antimicrobial Agents and Chemotherapy 55(6), 2648-2654.)
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Key words: Food Microbiology, foodborne pathogens, biofilm tolerance to disinfection, antimicrobial inhibitory activities (MIC determination, antimicrobial bactericidal activities (MBC determination), bacterial stress adaptation
Florence Dubois-Brissonnet coordinates research programs on adaptation and persistence of foodborne pathogens in food processing environments. She has developed an expertise on bacterial membrane adaptation under stresses through characterization of membrane fatty acid composition. Among others, this specific physiological effector is used to better understand the adaptation of pathogen biofilms and their resistance to disinfectants and natural antimicrobial compounds.
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